Major Collision Investigation Unit detectives are investigating a fatal collision in Violet Town on 19 September.
Two trucks collided on the Hume Freeway just after 2pm.
Both male drivers were airlifted to hospital with life-threatening injuries.
One of the drivers, a 56-year-old Maldon man, has died in hospital this evening.
The other driver, a 53-year-old man from Parkes in New South Wales, remains in hospital in a critical condition.
The Hume Freeway is expected to have significant traffic delays for some time.
The exact circumstances surrounding the collision are being investigated.
Anyone with information, dashcam/CCTV footage is urged to contact Crime Stoppers on 1800 333 000 or submit a confidential report online at www.crimestoppersvic.com.auExternal Link.