Dr David Martinez-Martin from the School of Biomedical Engineering. Image: University of Sydney
Cells, the most basic units of life that form all living organisms, have long guarded their secrets, but now an international team from the University of Sydney, ETH Zurich and the University of Basel has uncovered some of their secrets through the development of a world-first technique.
Scientists know that cells grow but it was commonly thought that they grow linearly or exponentially in size, before dividing.
Now, in a paper published in co-led by University of Sydney physicist , using a nanotechnology technique called ‘inertial picobalance’, scientists have identified that at the single cell level, yeast grow in sequential intervals or segments of linear growth (constant growth rate). At each interval yeast cells switch to faster or slower growth – a ‘gear-like’ tendency.
The research was performed with saccharomyces cerevisiae, a unicellular yeast organism fundamental in the production of bread, beer, wine and pharmaceuticals. The protein-encoding genes of many types of yeast mirror genes in animal cells, making its behaviour key to understanding human disease.
Notably, the behaviour found in yeast differs significantly from that of animal cells (including human). It was not until 2017 that also using picobalance, Dr Martinez-Martin and colleagues first observed that the mass of living mammalian cells fluctuate intrinsically – they ‘yo-yo’ in size.
Dr Martinez-Martin and his colleagues first observed that the mass of living mammalian cells fluctuate intrinsically. University of Sydney.
“We have uncovered processes that challenge models in biology that have been central for decades,” said Dr Martinez-Martin. “The behaviours we have identified in cells from fungus and animal kingdoms provide strong evidence that cells have different strategies to regulate their mass and size, paving the way to better understand how they can accurately form and reform complex structures such as the eyes, brain and fingers in our bodies.”
A recent mathematical model published in by Dr Martinez-Martin also offers fresh insight into the meaning of this once-secretive cellular flux.
“Another of our recent studies has found that while cell mass fluctuations have been detected in single mammalian cells, they can be perfectly viable in organisms comprised of many mammalian cells, including humans. Our modelling suggests that the body’s cells don’t all swell and decrease at the same time – instead they give and take from each other, maintaining an adequate distribution of the body’s mass and volume,” he said.
“Mass fluctuations may be used by cells to regulate cellular functions such as metabolism, gene expression, proliferation and cell death, by means of altering the concentration and crowdedness of chemical cellular components.”
Dr David Martinez-Martin in the lab. Image: University of Sydney.
The model also suggests that mass fluctuations allow cells to communicate, both by acting as biomechanical signals through volume fluctuations, and through the exchange of water and molecules.
“I believe this could be a fundamental mechanism which may help cells locate and communicate their position within an organism,” Dr Martinez-Martin said. “Therefore, it could be incredibly important, because it could allow cells to identify and serve their distinct role and purpose in the body.”
“Researchers believe that a better understanding of how cells change their mass and size over time, as well as dysregulation of this process (when cells change their size atypically), could be the key to developing the next generation of diagnostics and treatments for a range of diseases, such as cancer, diabetes and cardiovascular disease.”
About inertial picobalance – the technique used in the discovery
Part of the technology. Image: University of Sydey.
Dr Martinez-Martin, who has been recently distinguished by the , is the principal inventor of inertial picobalance, a new technology that measures the mass of single or multiple living cells in real-time, enhancing the understanding of cell physiology. The technology is currently being commercialised by the
In a published in 2017, using inertial picoblance, Dr Martinez-Martin and his colleagues discovered that the mass of living mammalian cells fluctuates intrinsically by one to four percent over seconds, largely due to water entering and exiting cells.
Using this technique, they were also able to observe cells infected with the vaccinia virus (a virus from the poxvirus family). The infected cells showed different mass behaviour over time than non-infected cells, potentially enabling a new way of detecting viral infections.
DISCLOSURE
The research was funded by the University of Sydney, the NCCR Molecular Systems Engineering, the Innosuisse (project 28033.1 PFNM-NM), the Swiss Nanoscience Institute Basel, and ETH Zurich.
David Martinez-Martin, Sascha Martin, Christoph Gerber and Daniel J Müller have filed two patents related to the picobalance technology and its applications (, ). David Martinez-Martin, Sascha Martin, Gotthold Fläschner and Daniel J Müller have filed a patent related to an environmental chamber used in this research (). David Martinez-Martin, Gotthold Fläschner and Daniel J Müller have filed a patent related to cantilevers to measure the mass of cells (). Gotthold Fläschner and Jonathan D. Adams are currently working with Nanosurf AG to commercialise the picobalance and related technologies.
